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生理信號測量

光遺傳學記錄分析系統

時間:2016-09-19來源:本站作者:玉研儀器

詳細介紹

光遺傳學研究系統利用LEDs光源對動物一個或者多個具體腦區的經過視蛋白修飾的神經元進行光學刺激。

光遺傳學研究系統可以整合到Pinnacle的腦電/肌電(EEG/EMG)研究系統,同步記錄睡眠或者癲癇活動。光遺傳學研究系統同樣與Pinnacle數據采集系統和Sirenia?軟件兼容。

系統內置一個光遺傳學的接口模塊,可與Pinnacle的三通道或四通道數據采集系統整合在一起工作,提供精確的時間和光照控制。模塊連接到一個電子轉換器和探頭。系統配備的LED光纖探頭兼容標準的套管放置技術,因此不需要光學轉換器。有四種探頭可選:1-或者2-通道光刺激,用于行為學研究;1-通道光刺激+EEG/EMG記錄,用于睡眠研究;1-通道光刺激+EEG腦電記錄,用于癲癇研究。

光遺傳學研究系統的主要優勢:

(1)高精度時鐘保證了由本模塊控制的所有事件的時間精度小于10us
(2)與Pinncale數據采集和分析系統的通訊接口
(3)對LED光纖探頭的電流和脈沖輸出的精確時間控制
(4)3個數字輸入,1個數字輸出通道
(5)2個獨立的外部刺激輸入通道

應用范圍: 睡眠、學習記憶、癲癇、行為學研究等.

 
OPTOGENETIC SYSTEM is designed to optically stimulate opsin-modified neurons in one or more specific brain regions using LEDs. It can be integrated into Pinnacle’s EEG/EMG systems to simultaneously record sleep or seizure activity. The system is also compatible with all Pinnacle Data Acquisition systems and Sirenia? software.

 
1. The Optogenetic Interface Module (OGIM) provides precise timing and illumination control of the LED fiber probe.
2. Our 8401-HS data conditioning and acquisition system (DCAS) performs secondary amplification and filtering before sending data to Pinnacle’s Sirenia Acquisition software of collection via USB.
3. The low-torque commutator, which is mounted above the cage, allows for unencumbered freedom of movement.
4. Electrophysiological signals are amplified and filtered at the head of the animal using our headstages. The mouse headstage contains up to 2 connectors to optogenetic LED probes and connects to a headmount via a friction fit.
5. Prefabricated headmounts reduce surgery time and provide ready-to-insert EMG leads.
6. An optional, SYNCHRONIZED VIDEO SYSTEM is available.
 
 

LED FIBER PROBES

Pinnacle’s optogenetic stimulation is based on LEDs coupled to 200/250 μM fiber optic cable with a minimum light output of 60mW/mm2. The LED fiber probes are implanted using a guide cannula and standard stereotaxic techniques. The LED fiber probe assembly plugs directly into a 2 mm electrical header on the headstage, removing any requirements for an optical commutator.

Custom LED fiber probes are available upon request.
 

SIRENIA SOFTWARE MODULES

Our optogenetic systems come with Pinnacle  basic acquisition and analysis software and is available for free on our website. Additionally, we offer packages that automate the sleep scoring and seizure identification processes. Unlock these powerful tools for rapid data analysis by upgrading to our Sirenia  Seizure Pro or Sirenia Sleep Pro software.
  
SIRENIA    SEIZURE PRO is an advanced analysis package that provides a platform for quickly identifying and analyzing user-defined seizure events over a given time period.  


  
SIRENIA SLEEP PRO reduces scoring time by automating the process with tools such as cluster scoring, threshold scoring, hypnograms, and spectral analysis.  

  
SIRENIA    X-Y TRACKING accurately detects and analyzes animal movement within a cage. Track locomotor behavior in real time or in previously recorded video data. Our software is compatible with tethered and wireless animals.

 

 

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